A general purpose nutritious agar base. This medium was first used for the isolation of dental pathogens. With the addition of 7% defibrinated blood the medium will support the growth of a wide range of fastidious organisms, the phosphate buffer will help neutralise the acids produced from the utilisation of glucose and thus maintain viability. The medium is not recommended for the determination of haemolytic reactions because of the glucose content.
Brain-Heart Infusion Mixture17.5
Method for reconstitution
Weigh 49 grams of powder, disperse in 1 litre of deionised water. Allow to stand for 10 minutes then swirl to mix. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 47°C then pour into Petri dishes.
Pale Straw colour, clear gel.
pH:7.4 ± 0.2
Minimum Q.C. organisms:
S. aureus WDCM 00033
E. coli WDCM 00090
Storage of Prepared Medium:
Plates – up to 7 days at 2-8°C in the dark. Capped container – up to 3 months at 15-20°C in the dark.
Surface, streaking out to single colonies.
Time and temperature to suit specimen/organisms.