This medium is a selective isolation medium for Taylorella equigenitalis the causative organism of contagious equine metritis. The medium is a sugar free base with a mixture of high grade casein and soy peptones as nutrients and with L-cystine and sodium sulphite as supplements and reducing agents. The medium is made selective with the additio of amphotericin (5 mg/ L) and trimethoprim (10 mg/L).
Streptomycin (200 mg/L) can also be used but sensitive variants of T. equigenitalis have been described.
Method for reconstitution
Weigh 37.5 grams of powder, disperse in 1 litre of deionised water. Allow to soak for 10 minutes, swirl to mix then sterilise by autoclaving at 121°C for 15 minutes. Allow to cool to 80°C, add 50ml of sterile horse blood and allow to‘chocolate’. Further cool to 47°C before adding antibiotic selective agents. Mix well and po ur into Petri dishes.
Chocolated Blood Agar.
7.3 ± 0.2
Minimum Q.C. organisms:
Storage of Prepared Medium:
Plates – up to 7 days at 2-8°C in the dark.
Surface, streaking out for single colonies.
37°C in 10% CO
for 2-3 days.