A medium recommended by the Harmonised European Pha
rmacopoeia for the cultivation of a wide
range of micro-organisms. Conforms to USP/EP/JP per
formance specification. The medium is also
commonly referred to as tryptone (or tryptic) soy b
roth and abbreviated to TSB. Enzymatic digests
of casein and soya bean act as a source of nitrogen
and glucose is a carbon source in the form of a
fermentable carbohydrate. Sodium chloride maintains
the osmotic balance and dipotassium hydrogen
phosphate acts as a buffering agent.
Pancreatic digest of casein
Papaic digest of soya bean
Dipotassium hydrogen phosphate
Powder: fine, free-flowing, homogeneous, buff
Finished medium: Straw, clear to slight haze
7.3 ± 0.2
Refer to appropriate SDS
Method for reconstitution
Disperse 30 grams of powder in 1 litre of deionised
water. Allow to soak for 10 minutes then swirl to
mix. Distribute into suitable vessels and sterilise
at 121°C for 15 minutes.
According to the sterility protocol defined in the
Harmonised European Pharmacopoeia the samples
are incubated in portions of the medium at 20-25°C
for 14 days. No growth of micro-organisms is
required for a sterility pass.
According to the growth promotion test defined in t
he Harmonised European Pharmacopoeia
subtilis, Candida albicans and Aspergillus brasilie
are inoculated (with not more than 100 CFU)
and incubated for not more than 3 days in the case
of bacteria, and not more than 5 days in the case
of fungi. The media is suitable if a clearly visibl
e growth of the micro-organisms occurs.
The media is also used for the preparation of the t
Pseudomonas aeruginosa and Bacillus subtilis.
Refer to the specific strain and preparation for
incubation time and temperature.
The media is also used for sample preparation and p
re-incubation for the test for specified micro-
organisms. Refer to individual guidelines for each
Dehydrated culture media: 10-25
C away from direct sunlight.
Up to 7 days at 2-8°C in the dark
Minimum Q.C. organisms
Growth is indicated by turbidity, refer to specific
guidelines as defined in the Harmonised European
European Pharmacopoeia 9