Bevis modified Mackey and Sandys original medium by introducing a double indicator to improve the differentiation of lactose and non lactose fermenting coliforms, staphylococci and streptococci. The swarming of Proteus spp. is inhibited. Lab M C.L.E.D. will grow many of the more demanding streptococci of Lancefield gr oups A, B, C, G and F. This medium may not grow
spp. or halophilic
Balanced Peptone No. 1
Agar No. 1
Method for reconstitution
Weigh 36 grams of powder, disperse in 1 litre of de
ionised water. Allow to soak for 10 minutes, swirl
to mix then sterilise by autoclaving
for 15 minutes at 121°C. Cool to 47°C and mix befor
Green/blue, clear gel.
7.5 ± 0.2
Minimum Q.C. organisms:
Storage of Prepared Medium:
Plates – up to 7 days at 2-8°C in the dark.
Surface inoculation, either streaking for single co
lonies or spread evenly over entire surface for col
37°C for 24 hours aerobically.