A medium used for the performance and confirmation
of the Presumptive Test for members of the coliform
group in water and dairy
Method for reconstitution
Weigh 13 grams of powder, disperse in 1 litre of de
ionised water, heat to dissolve then distribute int
o bottles with Durham tubes. Sterilise
by autoclaving at 121°C for 15 minutes.
Straw coloured, clear.
6.9 ± 0.2
Minimum Q.C. organisms:
Storage of Prepared Medium:
Capped containers – up to 3 months at 15-20°C in th
See methods for standard techniques.
35°C aerobically for 48 hours.
Coliforms are presumptively identified by their abi
lity to ferment lactose and produce gas within 48 h
ours at 35°C.
American Public Health Association. (1975). Standar
d Methods for the examination of water and waste wa
ter, 892. Washington. United
States Pharmocopeia, XXI, 1985.