Listeria Express Enrichment Broth (LEE Broth) is aselective enrichment broth for the detection of Listeria
. Developed to give improved growth rates of
over traditional selective enrichment
media, LEE Broth enhances the expression of target
antigens for most commercially available
immunological test kits/methods whilst maintaining
adequate suppression of potential non-target
organisms. Selective components are blended into th
e powder, removing the requirement for
Compared with more traditional methods, immunologic
al tests, such as ELISA, require relatively
high levels of target organisms to achieve a reliab
le positive result. LEE Broth has been specifically
designed to stimulate growth from low numbers to th
e required high levels within a 24 hour period.
Therefore, LEE Broth offers an excellent choice for
laboratories employing immunological test
methods for detection of
in food products.
Lab M LEE Broth can be used as an enrichment broth
prior to plating on selective media such as
Harlequin™ Listeria Chromogenic Agar ISO (HAL010 /
PIN001); as secondary selective enrichment
medium following primary enrichment in, for example
, Half Fraser Broth (LAB211 or LAB164); or
as the enrichment step of a rapid method e.g. ELISA
, lateral flow device, PCR.
Powder: fine, free-flowing, homogeneous, buff
Finished medium: clear, dark straw liquid with yell
7.2 ± 0.1
Xi – Irritant
Method for reconstitution
Disperse 46.2g of powder in 1 litre of distilled wa
ter. Allow to soak for 10 minutes, swirl to mix
and dispense into suitable containers. Sterilise by
autoclaving for 15 minutes at 121
C. Allow to
cool to ambient temperature prior to use.
Incubate at 30
C + 1
C for 24 + 1 hour (for optimum performance check at
24 hours precisely).
Alternative incubation temperatures may be used if
flagella are not the target antigen.
Grams per litre