A liquid medium of low pH for the growth of yeasts
and moulds, typically employed as part of sterility
testing protocols for various
products. The high carbohydrate content of the medi
um ensures rapid growth of yeasts and moulds.
Method for reconstitution
Weigh 20g of powder and disperse in 1 litre of deio
nised water. Allow to soak for 10 minutes, swirl to
dissolve and dispense into final
containers. Sterilise by autoclaving at 115°C for 1
Pale brown/straw, clear broth.
5.4 + 0.2
Inoculate samples direct into tubes of broth accord
ing to the particular method being employed.
25°C (or 37°C) for up to 7 days aerobically, depend
ing upon protocol used. Subculture turbid tubes ont
o solid media for
identification of growth.
Minimum QC organisms:
Galloway, L.D. and Burgess, R. (1952) Applied Mycol
ogy and Bacteriology, 3rd ed, Leonard Hill, London
pp 54 & 57.