An osmotically controlled solution which is an
alternative to, and a replacement for, 1/4
strength Ringer’s Tablets (LAB100Z). The presence o
f a low level of peptone lessens the
physiological shock normally experienced by bacteri
al cells when they are introduced to a
diluent such as Ringer’s Solution. The level of pep
tone is such that multiplication of the
organisms is not possible in the time in which the
sample will be present in the diluent (1-2
hours). This formula is recommended by ISO 6887: BS
Powder: fine, free-flowing, homogeneous, buff
Finished medium: clear, colourless liquid
7.0 ± 0.2
N.B. The absence of any buffer results in the produ
ct taking on the pH of any sample added.
NR – Not regulated
Method for reconstitution
Weigh 9.5 grams of powder and disperse in 1 litre o
f deionised water. Allow to soak for 10
minutes, swirl to mix and if required, heat gently
to dissolve. Distribute into final containers.
Sterilise by autoclaving for 15 minutes at 121
Dehydrated culture media:
C away from direct sunlight.
Capped containers – up to 3 months
at 15-20°C in the dark.
Minimum Q.C. organisms
Straka, R.P. and Stokes, J.L. (1957). Rapid destruc
tion of bacteria in commonly used diluents
and its elimination. Appl. Microbiol.
ISO 6887-1:1999 Microbiology of food and animal fee
ding stuffs — Preparation of test samples,
initial suspension and decimal dilutions for microb
iological examination – Part 1: General rules
for the preparation of the initial suspension and d