POLYMYXIN for the isolation of
Suitable for the preparation of LAB
73 Bacillus cereus Medium
(P.R.E.P.). The addition of X073 sterile egg yolk emulsion is also
8mg/litre = 64,000i.u/litre
Add 1 vial X074 to 500ml medium
Rehydrate contents o
f vial with 5ml of sterile deionised water. Add
aseptically to sterilised medium cooled to 47°C together with egg
emulsion, mix gently and pour.
-organisms in Food. Ed. Thatcher, F.S., Clarke, D.F. published
by Univ. of Toronto Press.
POLYMYXIN B for the isolation of
The addition of X073, Egg Yolk Emulsion, is also required. For
addition to LAB 193, PEMBA
Add 1 vial X193 to
Rehydrate contents of vial by the addition of 5ml of sterile deionised
water. Add aseptically to sterilised medium cooled to 47°C, mix
gently and pour.
CEFOPERAZONE, AMPHOTERICIN for the isolation of
spp. from clinical, environmental and food
Suitable for use with LAB 112 Campylobacter Selective Medium
(blood free) or with blood agar media. Incubation at 37°C gives better
results than at 42°C and is generally more convenient.
Add 1 vial X112 to 500ml medium
Add 1 vial X212 to 1 litre medium
Rehydrate contents of vial with 5ml of sterile deionised water. Add
aseptically to sterilised medium cooled to 47°C, mix gently and
Bolton, F.J., Hutchinson, D.N., Parker, G. (1988). Reassessment of
Selective Agars and Filtration Techniques for Isolation of
Campylobacter Species from Faeces. Eur. J. Clin. Microbiol. Infect.
Dis. 7: 155
TRIMETHOPRIM, to make
Skirrow’s medium for the isolation of
Suitable for use with LAB001 Columbia Agar or other blood agar
bases, supplemented with lysed horse blood.
Add 1 vial of X214 to 1 litre of medium
Rehydrate contents of vial with 5ml sterile deionised water. Add
aseptically to sterilised medium cooled to 47°C, along with other
additives, mix well and pour.
Skirrow, M.B. (1977)
British Medical Journal 2 11