What is Prunella Vulgaris Extract?
Prunella vulgaris is one of the Chinese herbs used in Asia. Prunella Vulgaris is a perennial plant known as ‘self-heal’ in Western herbal medicine.
The herb prunella vulgaris (commonly known as self-heal) is one of the few natural treatments that has shown promise as both an oral and topical treatment. It is edible, and contains vitamins A, C, and K. It is also rich in flavonoids, which provides this perennial plant with anti-inflammatory, anti-microbial, and anti-viral activities.
Historically, prunella vulgaris has been used in mouthwash to treat conditions like sore throats and gum disease. It is also made into tea to treat internal problems like internal bleeding and diarrhea.
Chemical constituents of Prunella Vulgaris Extract
The whole herb contains prunellin, Oleanolic acid.also contains Rutin, Hyperoside, Ursolic acid, Caffeic acid, vitaminsC,D, carotene, tan-nin, alkaloids, camphor, fenchone.
The flowers contains Delphinidin,Cyanidin,d-Camphor, d-Fenchone.
ursolic acid， oleanolic acid,daucosterol，β-mayrin,tetra-cosanic acid,hexacosanic acid， octa-cosanic acid,triacontanic acid.
The leafs contains oleic acid，linolenic acid， myristic acid， palmitic acid， steraric acidlauric acid.
Benefits of taking Prunella Vulgaris Extract supplements:
According to some laboratory studies, prunella has many potential benefits, including anti-microbial, anti-viral, and anti-oxidant properties. Prunella may have activity against herpes simplex virus which could be of benefit in oral herpes and genital herpes. However, human studies are lacking therefore not much more can be said about the clinical uses of a prunella vulgaris supplement.
>NF-κB-Targeted Anti-Inflammatory Activity of Prunella vulgaris var. lilacina in Macrophages RAW 264.7.
(Source-Department of Agrofood Resources, National Academy of Agricultural Science, RDA, Suwon, Gyeonggi-do 441-853, Korea.)
Prunella vulgaris var. lilacina, a herbal medicine, has long been used in Korea for the treatment of sore throat, and to alleviate fever and accelerate wound healing. Although the therapeutic effect of P. vulgaris var. lilacina is likely associated with anti-inflammatory activity, the precise underlying mechanisms are largely unknown. Here, we sought to elucidate the possible mechanisms of the anti-inflammatory activity. We have investigated the anti-inflammatory activity of the various solvent fractions (hexane, butanol, chloroform and water) from the ethanol extract of P. vulgaris var. lilacina in activated macrophages. The hexane fraction exhibited higher anti-inflammatory activities, inducing inhibition of nitric oxide and prostaglandin E2 production as well as inducible nitric oxide synthase, cyclooxygenase-2, and tumor necrosis factor-α mRNA expression in response to lipopolysaccharide stimulation. Moreover, the hexane fraction from P. vulgaris var. lilacina significantly inhibited the activation of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and the nuclear translocation of the NF-κB p50 and p65 subunits. These results indicate that P. vulgaris var. lilacina has an anti-inflammatory capacity in vitro, suggesting that it could be a potential source of natural anti-inflammatory agents.
>Effect of Chinese herbal medicine extracts on cell-mediated immunity in a rat model of tuberculosis induced by multiple drug-resistant bacilli.
(Source-Department of Pathogen Biology and Immunology, Anhui University of Science and Technology, Huainan 232001, PR China.)
Drug-resistant Mycobacterium tuberculosis poses a major threat to public health. The present study aimed to investigate the effects of Radix Ranunculi Ternati, Radix Sophorae Flavescentis, Prunella Vulgaris L. and Stellera Chamaejasme L. extracts on cell‑mediated immunity in a rat model of tuberculosis (TB) induced by multiple drug-resistant bacilli. The bacterium was isolated from patients infected with pulmonary tuberculosis. The immunological response in humans following infection with Mycobacterium tuberculosis involves a number of cytokines, including IFN-γ and IFN-α, which are important for killing intracellular micro-organisms. T helper type 2 (Th2) cells express numerous cytokines, including IL-4 and IL-10, which mainly participate in humoral immunity and induce the phagocytosis of extracellular bacteria and parasites. In the present study, rats were infected with multiple drug-resistant TB (MDR-TB) in order to establish an MDR-TB model. Peripheral blood mononuclear cells (PBMCs) were isolated and cultivated, and the serum levels of IFN-γ, IL-4, IL-10 and IL-12 were examined by ELISA. The mRNA expression levels of certain cytokines in PBMCs were additionally detected using RT-PCR. The serum levels of IFN-γ in the Radix Ranunculi Ternati, Radix Sophorae Flavescentis, Prunella Vulgaris L. and Stellera Chamaejasme L. groups were 2.01±0.73, 1.92±0.56, 1.98±0.67 and 1.94±0.59 pg/ml; IL-4 levels were 6.01±1.46, 6.12±1.35, 6.47±1.46 and 6.15±1.44 pg/ml; IL-10 levels were 12.09±3.07, 12.45±4.01, 12.13±3.43 and 12.54±3.78 pg/ml; and IL-12 levels were 2.99±0.89, 2.75±0.84, 3.02±0.86 and 2.89±0.75 pg/ml, respectively. These differences were significant compared with the model group (P<0.05). RT-PCR analysis revealed a significant increase in the levels of IFN-γ and IL-12, and a significant decrease in the mRNA levels of IL-4 and IL-10 (P<0.05). These results indicated that the extracts of Radix Ranunculi Ternati, Radix Sophorae Flavescentis, Prunella Vulgaris L. and Stellera Chamaejasme L. are capable of enhancing cell‑mediated immunity in rats by upregulating the levels of genetic transcription. This may explain the observed therapeutic effect of Chinese herbal medicines in the treatment of MDR-TB.
>Effects of triterpenic acid from Prunella vulgaris L. on glycemia and pancreas in rat model of streptozotozin diabetes.
Zhou QX, Liu F, Zhang JS, Lu JG, Gu ZL, Gu GX.
(Source-Institute of Pediatric Medicine, Soochow University Affiliated Children’s Hospital, Suzhou, Jiangsu 215003, China.)
The effects of triterpenic acid from Prunella vulgaris L. (TAP) on diabetes and its mechanism are uncertain. The aim of this study was to investigate the effects of TAP on antihyperglycemic, antioxidant, and pancreas-protective in streptozotozin (STZ)-diabetic rats.
The diabetic model was produced by injection of 60 mg/kg STZ. Blood was drawn from the tail vein of rats after 72 hours. Rats with blood glucose ≥ 16.7 mmol/L were considered diabetic. Diabetic rats were randomly divided into four groups: (1) Diabetes rat (STZ), (2) Diabetic rats treated with 50 mg/kg of triterpenic acid from Prunella vulgaris L (STZ + TAP50), (3) Diabetic rats treated with 100 mg/kg TAP (STZ + TAP100), and (4) Diabetic rats treated with 200 mg/kg TAP (STZ + TAP200). Normal rats (n = 10) acted as the control group (NC). TAP was administered by the intragastric route once each day for six weeks. Body weight and the concentration of blood glucose (BG) were measured after three and six weeks. Fructosamine (FMN), malondialdehyde (MDA), and nitric oxide (NO), and the activities of nitric oxide synthase (NOS) and superoxide dismutase (SOD) in serum were determined after six weeks using commercially available kits following the manufacturer’s instructions. Pathologic changes in pancreatic β-cells were also investigated by microscopic examination after hematoxylin-eosin (HE) staining. The level of SOD mRNA in pancreatic β-cells was measured by polymerase chain reaction (PCR).
The levels of BG, FMN, NO, and MDA and the activities of NOS in serum in the four diabetes groups were significantly increased compared with the control group (P < 0.01). The activity of SOD in serum and the body weight was significantly decreased compared with the control group (P < 0.01). After administration of TAP to diabetic rats for six weeks, the body weight and the levels of BG, FMN, MDA, NO and the activity of NOS in serum decreased significantly compared with the STZ group in a dose-dependent manner. The activity of SOD in serum and body weight increased significantly compared with the STZ group in a dose-dependent manner. In addition, diabetic rats showed a significant decrease in SOD mRNA expression in pancreatic β cells. However, these changes were reversed by TAP. Histopathological examination also showed the protective effect of TAP on pancreatic β cells.
>Triterpenic acid from Prunella vulgaris L. has an anti-diabetic effect, by controlling blood glucose and antioxidants, and has a protective effect on the pancreas.
The inhibitory effect of Prunella vulgaris L. on aldose reductase and protein glycation.
(Source-Institute of Natural Medicine, Hallym University, Chuncheon 200-702, Republic of Korea.
To evaluate the aldose reductase (AR) enzyme inhibitory ability of Prunella vulgaris L. extract, six compounds were isolated and tested for their effects. The components were subjected to in vitro bioassays to investigate their inhibitory assays using rat lens aldose reductase (rAR) and human recombinant AR (rhAR). Among them, caffeic acid ethylene ester showed the potent inhibition, with the IC(50) values of rAR and rhAR at 3.2 ± 0.55 μM and 12.58 ± 0.32 μM, respectively. In the kinetic analyses using Lineweaver-Burk plots of 1/velocity and 1/concentration of substrate, this compound showed noncompetitive inhibition against rhAR. Furthermore, it inhibited galactitol formation in a rat lens incubated with a high concentration of galactose. Also it has antioxidative as well as advanced glycation end products (AGEs) inhibitory effects. As a result, this compound could be offered as a leading compound for further study as a new natural products drug for diabetic complications.
Prunella Vulgaris and cancer
Regulation mechanism of triterpenoid components from Prunella asiatica on phase II detoxifying enzymes in vitro and in vivo
(Source-Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Traditional Chinese Medicine, Nanjing 210028, China. )
To study the effects of triterpenoid components from Prunella asiatica on phase II detoxifying enzymes and protein expression in vitro and in vivo. Normal human bronchial epithelial (NHBE) cell model was used in vitro, and the mouse model of Kunming (KM) mice was used in vivo. CDNB assay was used to measure the activity of GST. NADPH and DCIP was used to detect the activity of NQO1. DTNB colorimetric assay was used to detect GSH. Western blot was use to detect the protein expression of NQO1. We found that triterpenoid components from P. asiatica could increase the activity of GST, NQO1 and GSH in NHBE cells and KM mice. NQO1 protein expression can also be increased in vitro. The study suggests that triterpenoid components from P. asiatica can prevent the lung cancer by regulating the body phase II detoxification enzyme activity and protein expression.
>Mouth and throat ulcers.
>Stomach upset and irritation.
>Disorders of the female reproductive system (gynecological disorders).
Side effects and safety of Prunella Vulgaris Extract
Not enough is known about the use of self-heal during pregnancy and breast-feeding. Stay on the safe side and avoid use.
Dosage of Prunella Vulgaris Extract supplement:
Many Prunella Vulgaris Extract 10:1 powder supplements recommend a dosage of around 1500 mg to 2000 mg.