Product Code: MID-61E
TDA Reagent for Tryptophan Deaminase Reaction. This test determines the ability or otherwise of bacteria to deaminate the amino acid L-Tryptophan to produce Indolepyruvic acid. L-Tryptophan is deaminated (removal of the amino residue from the amaino acid) to an alpha keto acid (indolepyruvic acid) and ammonia. The formation of Indolepyruvic Acid is demonstrated by the addition of ferric ions (ferric chloride) in the presence of hydrochloric acid (1,2). It has been postulated that the ferric ions react with the indolepyruvic acid in the presence of hydrazine compounds in the culture media to produce ferric hydrazone which is a deep red to brown in colour.
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