A nutritious broth base recommended for the mainten
ance and propagation of yeasts widely used in gene
insertion techniques. Glucose is
included to promote rapid growth.
Method for reconstitution
Weigh 50.0 grams of powder and disperse in 1 litre
of deionised water. Allow the mixture to soak for 1
0 minutes, swirl to mix and dispense
into final containers. Sterilise by autoclaving at
121°C for 15 minutes.
Dark straw, clear liquid.
6.5 ± 0.2
Minimum QC organisms:
Storage of Prepared Medium:
Capped containers – up to 3 months at 15-20°C in th
As per normal techniques, using a pure culture of s
train to be cultivated.
37°C aerobically for 16-18 hours.
Examine all tubes for turbidity, indicating growth.